EFFECT OF pH UPON PROTEOLYSIS BY PAPAIN
نویسندگان
چکیده
منابع مشابه
EFFECT OF pH UPON PROTEOLYSIX BY PAPAIN” BY SAM R. HOOVER
The existence of pH optima for proteolysis was early established, primarily through measurements of the initial rate of reaction as a function of pH. When synthetic substrates for proteolytic enzymes were discovered, it was found that the rate of digestion depended upon the amino acids of the peptide and their arrangement in the chain. We have investigated the effect of pH upon the hydrolysis o...
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The controlled digestion of human immunoglobulin M (IgM) (Patient Dau.) with papain in the presence of 0.002 M mercaptoethylamine resulted in the release of a basic subunit-like fragment which we designated IgM,. It resembled the subunit (IgM,) produced by mild reduction of IgM in the following ways. The molecular weight (186,000) and sedimentation coefficient (6.2 to 6.5 S) of IgM, were simila...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1947
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)35156-6